Design and Fabrication of a Multi-Chamber Thermal Gradient PCR Chip

• Main Authors: Hsin-Chih Chen, 陳信志
• Other Authors: Jyh-Jian Chen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/63985372842541121785

碩士 === 國立屏東科技大學 === 生物機電工程系所 === 101 === This study used micromachining process technology to fabrication a multi-chamber thermal gradient polymerase chain reaction（PCR）chip. Commercial software CFD-ACE+TM is utilized to simulation a H-type aluminum block structure, chip on aluminum block, two sides of the H-type aluminum block heating, at different times change heating zone temperature, make chip respectively produce PCR required three temperature zone, during annealing region two cartridge heater rods are set at two different temperatures, to achieve a linear temperature distribution of thermal gradient on the chip during the annealing step. PCR chip fabrication five reaction chamber, to enable it achieve high throughput PCR, heater rods are placed at two sides of the H-type aluminum block, while the top and one side placement fans. LabVIEW temperature control program is utilized to collocation MOSFET switch circuit underway heater rods and fans control, K-type thermocouples inserted into the reaction chamber as temperature measurement, while using infrared thermal imager measurement chip surface temperature distribution. The experimental result shows, when fans adopt one in one out mode to makes chip cooling, enable chip to achieve a linear temperature distribution of during the annealing step. The polycarbonate （PC） chip during experimental measurement conform this device required three temperature zone, have sufficient heating duration to perform PCR. Final this devices compared with commercial thermal cyclers underway PCR experimental, to prove feasibility of this system in biological experimental.