Adventitious shoot regeneration of eggplant interspecific hybrid

• Main Authors: Lun-Chang Sung, 宋倫彰
• Other Authors: Shih-Wen Chin
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/31162282829533295934

碩士 === 國立屏東科技大學 === 農園生產系所 === 101 === Eggplant (Solanum melongena L.) is a vegetable and medicinal plant good for human health; furthermore, eggplant is an important crop all over the world. A number of studies report the regeneration of large number eggplants through tissue culture; for example, researchers would add hormone in the tissue culture media to induce the plant regeneration. Many reports were also indicated that using different explants of eggplant such as the leaves, roots, cotyledons, hypocotyls, and nodes, to induce plant regeneration. However, the studies which induce regeneration of eggplant interspecific hybridization varieties with various light intensity condition were limited. Consequently, in our experiment will use leaf explants of eggplant treating with artificial hormones, such as Auxin (naphthaleneacetic acid, NAA), Cytokinin (6-benzyladenine, BA) and Thidiazuron (TDZ) and with different light intensity conditions to induce plant regeneration. In the study, the effect of leaf explants treated with NAA and BA to induce shoot regeneration eggplant interspecific hybridization varieties were not significantly. On the other hands, IV TDZ have higher efficiency shoot regeneration rates than NAA and BA. The in vitro shoot were obtained in MS medium supplemented with 0.1 mg/L TDZ, having an average shoot number of 4.94 ± 1.84 per leaf explants (not fix size) in normal light intensity (35μmol/m2/s) for 21 days; on the other hand, the leaf explants (5 mm2) cultured in MS medium supplemented with 0.1 mg/L or 0.25 mg/L TDZ which in vitro shoot number was more leaf explants than that none fixed size, having average 11.19 ± 2.54 and 12.30 ± 3.30 per explants, respectively. The best in vitro shoot induction was achieved on MS medium supplemented with 0.1 mg/L TDZ, with an average of 15.41±0.71 per leaf explants (0.5mm2) in low light intensity (13 mol/m2/s). In our experiment, histology analyzing indicated that the in vitro shoot was through the organogenesis adventitious shoot. Finally, the adventitious bud inducing from the leaf explants treated with 0.1 mg/L TDZ in low light intensity would be growing and development after subculture to MS medium with hormone free in the normal light intensity condition for 42days. Furthermore, the upper leaf explants have highest survival rates after subculture to the MS medium for 42 days. On the other hand, the adventitious shoot inducing from the leaf explants all of position wouldn’t be growing and development after subculture to the MS medium without any plant growth regulator for 42 days; moreover, that have lowest survival rate. We were fined the optimal light intensity and concentration of artificial hormones, TDZ, to induce leaf explants of eggplant regeneration. The future we can try to use TDZ to induce regeneration of other eggplant interspecific hybridization varieties in the future.