Ferulic Acid Enhances Peripheral Nerve Regeneration across Long Gaps

• Main Authors: Sheng-Chi Lee, 李勝吉
• Other Authors: Ming-Chang Wu
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/21137448587310837005

博士 === 國立屏東科技大學 === 食品科學系所 === 101 === Abstract Student ID: P9836002 Title of Dissertation: Ferulic Acid Enhances Peripheral Nerve Regeneration across Long Gaps Total page: 69 Name of Institute: Department of Food Science, National Pingtung University of Science and Technology Graduate date: Jun 20,2013 Name of student: Sheng-Chi Lee Advisor: Ming-Chang Wu The contents of abstract in this dissertation: This study investigated the effect of ferulic acid (FA) against peripheral nerve injury in vitro and in vivo. In the in vitro test, the effect of FA on viability of Schwann cells was studied. In the in vivo test, right sciatic nerves of the rats were transected, and a 15 mm nerve defect was created. A nerve conduit made of silicone rubber tube filled with FA (5 and 25 µg/ml), or saline (control), was implanted into the nerve defect, and their nerve regenerative abilities were evaluated. Results show that the number of proliferating Schwann cells increased significantly in the FA-treated group at 25 µg/ml compared to that in the control group. After 8 weeks of nerve transection, the FA-treated group at 25 µg/ml had a higher rate of successful regeneration across the wide gap, a significantly calcitonin gene-related peptide (CGRP) staining of the lamina I-II regions in the dorsal horn ipsilateral to the injury, and a significantly shortening of the latency and an acceleration of the nerve conductive velocity (NCV) of the evoked muscle action potentials (MAPs) compared with the controls. We also found the FA could significantly diminish the number of macrophages recruited in the distal portions of the transected sciatic nerves, which may explain its nerve growth-promoting capacity in this critically-sized nerve gap model. In summary, the FA may be useful in the development of future strategies for the treatment of peripheral nerve injury. Key words: Peripheral nerve regeneration; Ferulic acid; Silicone rubber conduit