Study of the relationship between antiproliferation activity and fungal immunomodulatory protein structure of Ganoderma tsugae

• Main Authors: Tzu-Wen Tsai, 蔡慈雯
• Other Authors: Ying-Tang Huang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/64571219615192981134

碩士 === 國立高雄海洋科技大學 === 海洋生物技術研究所 === 101 === The fungal immunomodulatory protein (FIP), a small molecular-weight protein, is purified from Ganoderma tsugae mycelia. It have been shown that FIP have immunomodulatory and anti-cancer effects. Our previous study also showed that FIP could inhibit growth of HL-60 and Jurkat leukemia cell lines. However, the molecular mechanism is unclear. In this study, we focused on the relationship between antiproliferation activity and FIP structure. Our results showed that FIP N-terminal 13 amino acids could induce cell aggregation, the others FIP residues (FIPΔN13) had cytotoxicity activity. Dimer and trimer of FIPΔN13s were constructed. Trimer of FIPΔN13 was exhibited more cytotoxicity and aggregation than dimer of FIPΔN13 and FIP. FIP could induce apoptosis through activation of caspase-9, however, dimer and trimer of FIPΔN13s induced apoptosis mediated by endoplasmic reticulum stress through activation of CHOP. Dimer and trimer of FIPΔN13s also could inhibit cancer cell line A549 proliferation. Based on our results, there is a relationship between number of FIPΔN13 and cytotoxicity. In the future, molecular mechanism of cytotoxicity induced by FIPΔN13 will be carried out.