Effects Of Saussurea Involucrate Extract Flavonoid Rutin And Its Metabolites on Anti-inflammatory

• Main Authors: Ya-Ping Chen, 陳雅萍
• Other Authors: Yi-Lin Chen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/90974159074345919875

碩士 === 國立宜蘭大學 === 生物技術與動物科學系生物技術碩士班 === 101 === Saussurea involucrate Kar.et Kir., which is a rare traditional Chinese medicinal herb. Rutin and Hispidulin are flavonoids of the flavonol type that are found plenty in S. involucrate, which had been reported to have anti-inflammation, anti-oxidation and anticancer activity. In previous studies, the gut microflora in the large intestine metabolize rutin to a variety of compounds that such as 3,4-dihydroxyphenylacetic acid (DHPAA), 3,4-dihydroxytoluene (DHT), 3-hydroxyphenylacetic acid (HPAA) and 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid, HVA). This study aimed to evaluate the anti-inflammatory effect of the metabolites of Rutin on lipopolysaccharide-stimulated RAW 264.7 and explore the mechanism of its action. RAW264.7 were pretreated with drugs for 30 min prior to incubation with LPS for 24 h. Anti-inflammatory activity was evaluated with reference to inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) gene expression. In addition, activation of MAPKs and NF-κB by Western blotting. Our results showed that DHT significantly suppresses LPS-induced production of nitric oxide (NO) and iNOS and COX-2 in a dose-dependent manner, however, low concentration doesn't causing cytotoxicity. It also reduced the generation of proinflammatory cytokines, such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α. Further study showed that DHT not decreased LPS-induced mitogen-activated protein kinases (MAPKs) activation, but it significantly reduced LPS-induced I-κBα degradation and inhibited the translocation of nuclear factor κB (NF-κB)-p65 from cytoplasm to nucleus. Taken together, the results suggest that DHT may exert anti-inflammatory effects in vitro in LPS stimulated RAW 264.7 macrophages through inhibition of NF-kB signal pathway activation.