The Protect effect of the Anti-epileptic Drug Levetiracetam on Glutamate-induced Cell Damage in Cortical Neuron

• Main Authors: CUEI-SIA, CHEN, 陳翠霞
• Other Authors: Sheau-Huei Chueh
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/95420076011289396770

碩士 === 國防醫學院 === 生物化學研究所 === 101 === In this studying we used rat cortical neuron as the Material to understand the effect of anti-epileptic drug Levetiracetam (Lev) on glutamate-induced cell damage. We got cortical neuron from 24 hours postnatal SD rat’s cortex, cultured with 10µM Ara-C in coverslip containing poly-D-lysine at last 3 days(DIV 3). We pre-treated Levetiracetam 300µM, than stimulated cells with glutamate to see if Levetiracetam could protect cell. The result exhibited Levetiracetam reduced glutamate-induced cell damage in cell death and the generation of ROS. We also found that pre-treated Levetiracetam can lower the glutamate-induced calcium influx. Then we added the calcium chelator EGTA to blocked calcium. We observed that after adding EGTA, the effect of glutamate-induced cell damage is disappeared. Thus, our concluded that Levetiracetam can protect cortical neuron to against glutamate-induced cell damage through the calcium- depend pathway.