| Summary: | 碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 101 === In this study, investigation to the differences between the adhesion forces and their gene expression for the rat adipocytes precursor cells (3T3-L1) were cultured in different hard and soft substrates which cells under the fluid shear stress stimulation. The fluid shear stress stimulation system with the man-machine interface controller can control the temperature, fluid flow, pH of medium and provides a stable environment for in vitro cultivation purposes.
Both hard and soft substrates for different proportions of PDMS were used in this study (silicone body: curing agent = 7:1 and 14:1 mix ratio). Both hard and soft substrates are hydrophobic materials which are no significant difference. The surface roughness of hard base (7:1) is larger than that of soft base material (14:1), but the roughness does not affect cell adhesion. In addition, adhesion force changes at different cultured time depends on the different growth conditions, it’s no obvious relationship with integrin.
In the shear stress stimulation, the direction of adipocytes growth under fluid shear stress stimulation presented perpendicular to the direction of fluid flow. This tendency is more obvious when the shear stress is greater and the cells also showed a more elongated. In addition, the fluid shear stress stimulation will directly affect cell growth situation.
For the integrin gene expression, the integrin gene expression is no difference for different cultured time under no fluid shear stress stimulation. The integrin (β3, β4) gene expression significantly increased the amount under lower shear stress (2 dyne/cm2) which is greater than that of higher shear stress (12 dyne/cm2). The relationship between cell adhesion force and integrin gene expression is negative at the same cultured time.
Keywords: adipocytes, fluid shear stress, cell adhesion force, integrin
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