Analysis of physiological responses toward silver nanoparticles application in Arabidopsis thaliana

• Main Author: 許祐宇
• Other Authors: Huey-Wen Chuang
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/96217938718643430593

碩士 === 國立嘉義大學 === 生物農業科技學系碩士班 === 101 === The products of nanotechnology mean that it made from the nanoparticle which the diameter between 1 and 100 nanometer (nm).These kinds of products are everywhere surrounding with us in daily lives. From all of the products, silver nanoparticles have been widespread used in a lot of places because of its antimicrobial effects, such as textile industry, postharvest of agriculture products, medical products. Although already been used in many areas, the effects of silver nanoparticles on plant physiology still remain unknown. In this experiment, we use Arabidopsis thaliana as an experimental model to examine the effects of silver nanoparticles treatment on plant growth and development. Our results indicated that silver nanoparticles of different shapes and concentrations induced various responses in plants such as plant growth, accumulation of anthocyanin and ROS molecule, induction of enzymes involved in photosynthesis and sugar synthesis. In addition, it has the ability to decrease the perception of ethylene, in which the ethylene signals transduction and response was decreased, but the oxidation stress was increased. Addition of thiamine decreased the oxidation stress caused by silver nanoparticles treatment, and improved root extension and decreased anthocyanin accumulation levels. In conclusion, results of this study indicate that silver nanoparticles treatment causes different responses to plants depending on its concentration and shapes. Induction of root length elongation caused by treatment is due to altered auxin sensitive in treated tissues.