| Summary: | 碩士 === 國立彰化師範大學 === 生物技術研究所 === 101 === Patients with chronic kidney disease (CKD) are increasing. Interventions such as controlling hypertension and specific pharmacologic options are recommended. While some isoflavonoids may have benefits in this regard. We design this study into two parts. Part I investigated the effect of isoflavonoids. Naringenin (a flavanon), catechin (a flavanol), and quercetin (a flavonol) and rutin (a flavonol rutinoside) were tried on doxorubicin-induced CKD in Sprague Dawley rat model. Results indicated quercetin to be the most effective therapeutic candidate with respect to renal edema, serum creatinine, HCT, cardiopathy, glomerular amyloidosis, erythrocyte depletion in bone marrow, collagen deposition, expressions of TNF-, cleaved caspase-3, PPAR, and serum insulin. But quercetin was only partially effective in restoring glomerular filtration rate, serum cholesterol, triglyceride, blood urea nitrogen (BUN), MDA, SOD. As for signalings, quercetin was completely effective in alleviating the cleaved caspase-3, being only partially effective in suppressing Bax and Bad, restoring Bcl-2, and rescuing DNA damages. The CKD status is unable to be ameliorated by naringenin, rutin, and catechin. Comparatively, quercetin may be a better therapeutic candidate. Part II studied the combinational effect of some clinical drugs (Nifedipine, Atorvastatin, Captopril) with quercetin. The results show that Atorvastatin alone owns the best anti-hyperlipidemia and antioxidative effect but Nifedipine alone exacerbate hyperlipidemia, high BUN and high creatinine. Moreover, Nifedipine further increases the apoptosis for CKD.
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