| Summary: | 碩士 === 國立中央大學 === 光電科學與工程學系 === 101 === In this thesis, a metal-assisted guided mode resonance (MaGMR) optical biosensor is demonstrated. We use the nanoimprint lithography to replace the traditional semiconductor lithography processes, using this method the yield rate can be increased and reduce production costs. Then, we use the aptamer to be the recognizing part and using the metal-assisted guided mode resonance (MaGMR) device to be the transducer, so the device is called aptasensor. After that, we also built a multi-channel detection system for MaGMR aptasensor, and the 15-mer and 29-mer thrombin aptamer was immobilized on the surface of MaGMR device as a recognizing ligand for thrombin detection.
This study is divided into three parts：simulation, process and detection. The part of simulation is mainly used rigorous coupled-wave analysis (RCWA) method to analyze the difference and sensitivity between MaGMR and GMR; it shows the MaGMR is a high sensitivity device. The part of process shows the home-made hot embossing nanoimprint system for MaGMR chip. Then we design and optimize the nanoimprint device of MaGMR. After that, we used atomic force microscopy (AFM) and scanning electron microscope (SEM) to inspect the structure of MaGMR; it can verify the feasibility of the hot embossing nanoimprint MaGMR. The part of detection is mainly built multi-channel detection platform. At first, we use sodium chloride solutions to check the bulk sensitivity of MaGMR. The background index varies from 1.3330 to 1.3705, it can calculate sensitivity and noise ratio. The experimental result is stable and repeatable. We utilized the surface modification methods to form a self-assembly monolayer (SAM) on the surface before the detection of thrombin. This study used electron spectroscopy for chemical analysis (ESCA) to verify the surface modification result. Finally, we achieve the thrombin sensing in concentration range from 0.25 to 1.5 μM, and verify the specificity of aptasensor.The limit of detection (LOD) is 0.12M and 0.16M for 15 and 29-mer antithrombin aptamer, respectively.
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