Using Ultrasonic Standing Wave Fields in Association with Self-Assembly Microspheres to Enhance the Efficiency of Molecular Transport in vitro

• Main Authors: Yen-Lin Wei, 魏延麟
• Other Authors: Yu-Hsiang Lee
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/92158623305191534515

碩士 === 國立中央大學 === 生物醫學工程研究所 === 101 === To enhance the cellular therapy efficacy and scale up the drug delivery system used, we aimed to develop a complex molecular delivery system comprising ultrasound standing wave fields (USWF) and microsphere techniques. In this study, CdSe/ZnS quantum dots (QDs) were used to imitate drug molecules and the QDs-coated polystyrene microspheres were prepared through layer-by-layer approach. The developed QDs-coated microspheres were characterized using microscopy and spectrofluorometry, and exhibited that 1) QDs can entirely cover the surface of microspheres with uniform distribution in a coverage rate of 1.0 pmole/cm^2, 2) QDs-covered microspheres exhibited similar optical properties with isolated QDs, and 3) the electrostatic interactions between QDs and microsphere surfaces were robust enough to resist mechanical stress induced by ultrasound. After determining the optimal USWF exposure time of 5 minutes in which the cellular viability was > 90% within 48 h, we examined the efficiency of microspheres internalization of the DH82 macrophages with and without USWF treatment using flow cytometry. Our results showed that the cells with USWF exhibited 1.2-fold (P < 0.01) and 1.3-fold (P < 0.01) higher than the group without USWF in terms of fluorescence-expressed cell number and fluorescence intensity from the cells, respectively. The system of USWF in association with microspheres developed in this study provided a feasible means for enhancement of molecular transport efficiency in vitro.