Identification of proteins with different expression in plasma from active systemic lupus erythematosus by gel electrophoresis and mass spectrometry

• Main Authors: Yi-Syuan Li, 李怡萱
• Other Authors: Shir-Ly Huang
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/51122181508880113785

碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 101 === Systemic lupus erythematous (SLE) exhibits an aggressive clinical phenotype with severe complications and overall poor prognosis. Common initial and chronic complaints include fever, joint pains, fatigue and temporary loss of cognitive abilities. These symptoms are so similar with other disease, so it is very difficult to diagnose and treat. The goal of this study is to identify significant different proteins between SLE patients compared to healthy people, the significant proteins may help to diagnose SLE patients. The SLE plasma samples which included male and 18 female patients, with average age 32.1±11.5 also diagnosed with renal involvement. The SLE plasma samples were collected from Cathay General Hospital, Taipei, Taiwan. The protein profile from patients was randomly compared to that from twelve healthy controls from 3 male and 9 female with average age at 41.9±12.2. The protein concentration in the plasma samples was quantified and the total protein was separated by one-dimensional and two-dimensional SDS-polyacrylamide gel electrophoresis. The up- and down-regulated proteins compared to healthy plasma (U-test, p < 0.5) were selected and cut from the gels and digested with trypsin for enzymatic analysis of protein using mass spectrophotometry for identification of proteomic analysis followed by the amino acid sequencing with mass spectrometry (MALDI-TOF/TOF and ESI-MS/MS). This experiments results a total of 15 proteins found, 9 proteins are up-regulated and 6 proteins are down regulated. In total of 15 proteins, nine proteins are published relation with lupus and six proteins are new.These new proteins in the SLE patients may contribute to find the new potential lupus biomarkers determine the precise role of newly identified SLE-related proteins pathogenesis and their function as biomarkers will require further study.