| Summary: | 碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 101 === Systemic lupus erythematous (SLE) exhibits an aggressive clinical phenotype with
severe complications and overall poor prognosis. Common initial and chronic complaints
include fever, joint pains, fatigue and temporary loss of cognitive abilities. These symptoms
are so similar with other disease, so it is very difficult to diagnose and treat. The goal of this
study is to identify significant different proteins between SLE patients compared to healthy
people, the significant proteins may help to diagnose SLE patients. The SLE plasma samples
which included male and 18 female patients, with average age 32.1±11.5 also diagnosed with
renal involvement. The SLE plasma samples were collected from Cathay General Hospital,
Taipei, Taiwan. The protein profile from patients was randomly compared to that from twelve
healthy controls from 3 male and 9 female with average age at 41.9±12.2. The protein
concentration in the plasma samples was quantified and the total protein was separated by
one-dimensional and two-dimensional SDS-polyacrylamide gel electrophoresis. The up- and
down-regulated proteins compared to healthy plasma (U-test, p < 0.5) were selected and cut
from the gels and digested with trypsin for enzymatic analysis of protein using mass
spectrophotometry for identification of proteomic analysis followed by the amino acid
sequencing with mass spectrometry (MALDI-TOF/TOF and ESI-MS/MS). This experiments
results a total of 15 proteins found, 9 proteins are up-regulated and 6 proteins are down
regulated. In total of 15 proteins, nine proteins are published relation with lupus and six
proteins are new.These new proteins in the SLE patients may contribute to find the new
potential lupus biomarkers determine the precise role of newly identified SLE-related proteins
pathogenesis and their function as biomarkers will require further study.
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