Characterization of five DEAD-box RNA helicase genes, RH2, RH6, RH22, RH42 and RH51 in Oryza sativa

• Main Authors: Yu-fu Chen, 陳玉夫
• Other Authors: 陸重安
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/62854765974083016306

碩士 === 國立中央大學 === 生命科學系 === 101 === Land plants are immobile and therefore encounter for various biotic stresses and abiotic stresses. Plants have to change in molecular, cellular and physical mechanisms to adapt those stresses. Temperature is the one of important factor for all of developmental stage of plant, form germination to reproduction. Low temperature inhibits seed germination, shoot elongation and overall metabolism and therefore cause to decrease agricultural productivity reduction. RNA helicase is motor protein witch can unwind of duplex RNA/RNA in an ATP-dependent manner. RNA helicases are involved in most of the basic cellular progresses including nuclear transcription, ribosome biogenesis, pre-mRNA splicing, nucleocytoplasmic transport, translation, mRNA decay and organall gene expression. DEAD-box RNA helicases belong to the helicase superfamily 2. The name is derived from the amino-acid sequence D-E-A-D (Asp-Glu-Ala-Asp) in the conserved motif II of RNA helicase family. Rice is one of the most important crops and also is a model plant for monocots. Base on amino acid sequence analysis, found the five homologues DEAD-box RNA helicase genes, OsRH2, 6, 22, 42 and 51, in rice that are similar to cold-stress response genes in Arabidopsis. The expression of five genes, showed that OsRH42 and OsRH51 are induced of temperature and salt stress in WT suspension cell. But the OsRH2, 6, 22 are consistive expression in various stresses treated cells. We further generated the RNAi knock-down transgenic lines for those DEAD-box RNA helicase genes. OsRH2 RNAi transgenic plant present plant height and internode were shorter than WT, and seeds fertility are also less than WT. Microscope analysis showed that pollen aborted rate of OsRH2 RNAi transgenic plants were higher than WT. Our results suggest that OsRH2 may have function in plant growth and seed development.