A Colorimetric and Fluorescence Dual-Function Probe for Detection

• Main Authors: Tsai, Jia-Chun, 蔡佳君
• Other Authors: Wang, Yun-Ming
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/07726215548921109195

碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 101 === We designed and synthesized a hydrophilic probe, 7-nitro-2,1,3-benzoxadiazol-4-yl thiocyanate (NBD-SCN), that can detect four specific biothiols [hydrogen sulfide (H2S), cysteine (Cys), homocysteine (Hcy) and glutathione (GSH)] on the basis of colorimetric and fluorescent methods. Especially, the probe can distinguish three biothiols by observing the remarkable change of the visible color via naked eyes and quantitative analysis utilizing UV-Visible spectrophotometer. H2S induce a distinct color change from colorless to violet and absorption bands shift to 530 nm. The relationship between absorbance and concentration of H2S presents an excellent linear correlation and shows a detection limit of 0.28 µM. Cys and Hcy induce a distinct color change from colorless to orange and absorption bands shift to 480 nm. GSH induce a distinct color change from colorless to yellow and absorption bands shift to 420 nm. In addition, NBD-SCN displays a high selectivity for Cys and Hcy in fluorescent enhancement. NBD-SCN exhibited 650-fold and 798-fold enhancement in fluorescence intensity by presence of 10 equiv. Cys and Hcy. The relationship between fluorescence intensity and concentration of Cys and Hcy presents an excellent linear correlation and shows a detection limit of 14.4 nM and 10.8 nM. The fluorescent images of biothiols in living cells have also been demonstrated.