Rapid resolving of bead and cell concentrations by silicon nitride micropore sieve device

• Main Authors: Hsu, Hsiao-Lin, 徐筱淋
• Other Authors: Sheu, Jeng-Tzong
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/62643462169621156369

碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 101 === In this research, silicon nitride micropore sieve was prepared and applied to resolve bead and cell concentrations via the measurement of the conductance across the pore. The micropore sieve was fabricated via microfabrication processes on the free standing silicon nitride membrane with the pore number ranging from 9 to 484. Electrical equivalent circuit model of the micropore sieve device was constructed and compared with the measurement data. Analytes were flowed through the micropores at a flow rate of 20 µl min-1 via a syringe pump. The AC sine-wave voltage (10 mV rms) at 10 Hz was applied between two Ag/AgCl electrodes across the micropores by a lock-in amplifier, and the ionic current was analyzed. Polystyrene beads and HeLa cells concentrations were characterized separately. Devices with different pore number were optimized and applied to resolve the beads/HeLa cells concentration. In 2 minutes, the device with 49 micropores exhibited the best resolving ability for polystyrene bead and HeLa cells concentration ranging from 102/ml to 106/ml.