Synthesis and analysis of self-assembly peptide supramolecular hydrogel biomaterial conjugated with gold nanoparticles

• Main Authors: Huang, Shun-Chiang, 黃舜強
• Other Authors: Ko, Fu–Hsiang
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/34199658716970636965

碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 101 === Recently, development of hydrogel nanocomposites offered tremendous scope for future biomaterial applications, including bioanalysis, chemical sensing, and drug delivery. Smart combinations of different nanostructured materials supported the development of multifunctional nanomaterials. In this study, synthesizing self-assembly peptide supramolecular hydrogel 2-Naphthylacetic acid- L-Phenylalanine- L-Phenylalanine- L-Cysteine (NapFFC) has been done and conjugated with gold nanoparticles (AuNPs) as a new and potential composite material. Finding stimuli-response properties for critical hydrogelation conditions such as concentration, pH, ionic strength, temperature, and incubation time were defined by visual. Further discussion for protection ability resulted from interaction between NapFFC and AuNPs was analyzed, and the results showed hydrogel and nanoparticles were complementary materials. Fibrous network and various kinds of helix structure resulted from self-assembly molecule blocks was observed and analyzed. Fluorescence intensity emitted at 335 nm was also helpful to obtain concentration of NapFFC under 60 μM within which self-quenching didn’t occur. Utilizing fluorescence quenching effect of AuNPs was quite sensitive and precise to measure the accurate amounts of molecules bound to the surface of AuNPs. This method was also used to detect quenching effectiveness of mercury ions in this study. Moreover, cell viability showed good biocompatibility. These analysis and results of composite material were attractive feature for exploitation in the field of biomaterials.