Extraction of cardiac action potential with microelectrode array

• Main Authors: Wu, Pei-Lin, 吳珮琳
• Other Authors: Sheu, Jeng-Tzong
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/73879418259630612157

碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 101 === The objective in this research is to set up a measurement platform which could record the electric signal called action potential from living cells. Then we can understand the communication of cells. However, there are many kinds of method to record the action potential. In this research, we use the microelectrode array （MEA） to capture the electrophysiological signals. Comparison to the traditional patch-clamp technique, it is non-invasive for cell. On the other hand, the microelectrode array could be used to impedance measurement. By the result of the impedance measurement, we know the characteristics of electrodes and the adhesion of cells. Because the electrophysiological signal is weak, the signal processing is by the lock-in amplifier then acquisition through NI-DAQ. Base on the above strategy, we demonstrate the recording of the electrophysiological signals by circular electrode which diameter is 20, 40, 60, 80, and 100 μm. Otherwise, the increase of contraction rate was measurement by our measurement system after the treatment of epinephrine.