Exercise training attenuates aging-induced cell apoptosis in SD rat hearts.

• Main Authors: Tsui-Hsien Hung, 洪翠舷
• Other Authors: Ying-Lan Tsai
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/31435920063829089695

碩士 === 國立體育大學 === 運動保健學系碩士班 === 101 === Backgrounds: Healthy problem is the major concern of aging population in present society. Exercise is considered one of the necessary methods to promote healthy in aged population. The effects and mechanisms against aging and heart protections in long-term exercise training are our main purpose in this research. Materials and Methods: We use intraperitioneal injection D-galactose (150mL/kgBW, 8 weeks) as an acute aging model and combined with or without the exercise training. Our exercise training prescription is swimming in warm water 60 min per day and five times per week. H &; E staining assay was applied in tissue analysis. DAPI / TUNEL staining assay was used to evaluate the apoptosis cells ratio in tissue slides. Proteins extracts were analyzed by western blotting. Results: Although the macroscopic phenomenon results showed the animal heart weight has no significantly change in the four groups. However the heart cell composition was disordered in intraperitioneal injection D-galactose as acute aging model then normal SD rats. Long-term exercise training had improved the disordered. Besides, the cardiac cell apoptotic ratio were significantly lower on long-term exercise training group by DAPI /TUNEL assay. Moreover, the proteins analysis observed higher expression of SIRT1-PGC1α-AMPK anti-aging signaling pathway in long-term exercise training animals. Conclusion: Our research results determined the situation of heart tissue senescence was similar between intraperitioneal injection D-galactose as an acute aging model and normal aging model. Long-term exercise training can enhance the SIRT1 anti-aging signaling pathways to protect heart tissue against aging. Our research results suggest that exercise can promote healthy and heart function in this aged population.