Study of the Property of PC12 Cells by Post-Cell-Removal Surface Morphology Profiling Technique

• Main Authors: Yan-Chang Chen, 陳衍暢
• Other Authors: You-Lin Wu
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/48030095898126970740

碩士 === 國立暨南國際大學 === 電機工程學系 === 101 === Detection of cell-substrate interaction is one of the methods for understanding cells properties. In this thesis, we tried to use atomic force microscopy to observe the post-cell-removal surface morphology profiles of pheochromocytoma 12 (PC12) nerve cells cultivated on 3-aminopropyltriethoxysilane (γ-APTES) surface, investigating the difference of the post-cell-removal surface morphology profiles of the PC12 cell before and after adding stimulus. Although the symptoms of Alzheimer's disease can be controlled medically, the disease itself is not curable. According to the report in the literature, it has been confirmed that in the brain of the patients with Alzheimer’s disease there is excessive amount of β-amyloid precipitation, and taking sesamin can soothe the symptoms of Alzheimer’s disease. Therefore, the aim of this work focuses on the Alzheimer's disease, investigating the influences of the stimulus on the post-cell-removal surface morphology profiles. In this thesis, live PC 12 cells were cultivated onγ-APTES modified SiO2 surface in culture mediums with no stimulus, withβ-amyloid, and with β-amyloid plus seasamin, respectively, and the post-cell-removal surface morphology profiles were obtained after the cells were cultivated for a certain amount of time. The changes of the post-cell-removal surface morphology profiles were analyzed before and after adding the stimulus.