A comparative proteomics analysis of peritoneal dialysate before and after the occurrence of peritonitis episode by mass spectrometry

• Main Authors: Sing-SingTing, 陳成欣
• Other Authors: Pao-Chi Liao
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/11559201736443628725

碩士 === 國立成功大學 === 環境醫學研究所 === 101 === Peritoneal dialysis (PD) is one of the therapeutic options for end-stage renal disease (ESRD). The peritoneal membrane is immersed in a high glucose concentration of the peritoneal dialysate which in long term may cause structural and functional damage to the peritoneal membrane. Peritonitis is one of the major complications of PD which will accelerate the damage of peritoneal membrane by increasing the peritoneal permeability and decrease the ultrafiltration efficiency. Finally it will cause the peritoneal membrane dysfunction and the patient may have fluid overload related complications and have to switch to hemodialysis. Thus, this study aim to enhance our understanding of the protein changes in peritoneal dialysate after the occurrence of peritonitis. In this study, we performed 2DE on 12 pairs of peritoneal dialysate before and after the peritonitis and approximately 400~600 spots were detected. Among these spots, 136 spots were excised, tryptic digested and identified by RP-nano-HPLC-ESI-MS/MS. 41 proteins with high levels of confidence were identified. Among these, ten proteins were significantly differentially expressed in peritoneal dialysate before and after the peritonitis, Western blot and ELISA validation were done. The present study was designed to develop a proteomic map of proteins before and after the peritonitis occurrence. These proteins are not the new biomarkers but they may indicate a situation for possible drug treatment, it may be the predictors of peritonitis which required validation study in future.