Self-assembly and FRET of Dye-functionalized Janus Particles

• Main Authors: Keng-MIngCheng, 鄭耿閔
• Other Authors: Changshu Kuo
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/72367369183460555303

碩士 === 國立成功大學 === 材料科學及工程學系碩博士班 === 101 === In this research work, asymmetric Janus particles were fabricated from the investigation of the self-assembly and the fluorescence resonance energy transfer (FRET). Janus particles cored with 500 nm silica colloids were functionalized with amino groups on one of their hemispheres, followed by the attachment with fluorescence dye, Marina Blue. These Janus particles in the submicron scale were carefully fabricated and characterized. Commercially-available polyacytlonitrile (PAN) particles with 100 nm in diameter and carboxylic surface-functionalities were employed as the self-assembly counterpart. These PAN particles were pre-loaded with another fluorescence dye, Chromeon 470, served as the FRET acceptor, while the Marina Blue Janus particles were utilized as the FRET donor. Self-assembly of these particles in an aqueous solution were initiated by the positively-charged asymmetric 500 nm Janus particles and the negatively-charged 100 nm PAN particles. The assembly and the cluster sizes were monitored by the dynamic light scattering. Meanwhile, the formation of the assembled clusters shortened the distance between the donor and acceptor, that was in fact the most crucial parameter for the FRET. Photoluminescence for both excitation and emission spectra indicated the successful FRET as these two particles process the self-assembly.