Efficacy study of non-thermal micro-plasma for promoting wound healing

• Main Authors: Yi-ChengWang, 王奕程
• Other Authors: Jiuun-Der Liao
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/62669866282157885090

碩士 === 國立成功大學 === 材料科學及工程學系碩博士班 === 101 === Currently, wound healing and skin regenerations are one of the major public health concerns which required considerable method and takes time. The laser irradiation is the frequently using method for the facial problems such as wrinkle, scar, pigmentation and acne. In addition, it could be promotes the remodeling of dermal-epidermal junction and need 4~12 weeks for complete recovery from wound healing and skin regeneration. In general, the common clinical prescription of the laser irradiation of the wound has been used by healing ointment such as aqurphor healing ointment (AHO), biafine topical emulsion (BTE), polysporin and neosporin, etc. However, healing ointment has might feel uncomfortable, tingling and allergy problem for some patients which indicates the urgent need to develop effective techniques to overcome the above problems. In the recent years, plasma therapy has attracted widespread interest which providing more advantage such as contact-free, painless, non-allergy and with antibacterial effect. In this present work, we are introducing non-thermal micro-plasma techniques which significantly inducing skin remodeling dermal-epidermal junction and promoting wound healing. Herein, the animal modeling was divided into three conditions such as non-treatment (NT), plasma treatment once (PT1) and plasma treatment three times (PT3). The non-thermal micro-plasma was generated from radio frequency and working distance between skin surface and micro-plasma was 4 mm. The optimized micro-plasma plume temperature 37°C was controlled by adjusting applied power and nitrogen gas insert. The plasma species was investigated with optical emission spectroscopy (OES) which resulted that the NO, OH, O, and Ar were major species in the non-thermal micro-plasma system. The reduction percentage of wound area at the 21 postoperative days (POD) was found to be 80%, 90% and 100% for NT, PT1 and PT3 respectively in presence of nitrogen 0.1%. In addition, the highest intensity of NO species was significantly increased by the laser irradiated wound healing. The wound healing quality assessments were studied by the optical coherence tomography (OCT), histochemistry and immunohistochemistry staining. The remodeling of dermal-epidermal junction, reepithelialization and well-developed granulation tissues were observed at 7 POD for PT1, PT3 group and 14 POD for NT group. The quantification of OCT image intensity at 14 POD and blood flow intensity at 21 POD results suggest that NT, PT1, PT3 group increased 49.6%, 87.6%, 87.2% and 37.0%, 35.2%, 60.4% by comparing with 1 POD intensity. Theses evidences proved that this novel non-thermal micro-plasma technique promising to be used as a fast and effective treatment for laser irradiated wound healing and remodeling the dermal-epidermal junction.