| Summary: | 碩士 === 國立成功大學 === 生物醫學工程學系 === 101 === Parkinson’s disease (PD) is known to be caused by insufficient release of dopamine (DA). Recording the DA level of animal’s brain could provide a direct evidence for evaluating novel treatments for PD in the acute or long-term studies. However, detection of DA in the biological system with high selectivity and sensitivity has been great challenge in electroanalytical research. Thus, there is a great need to develop a novel electrochemical method to selectively detect DA in neural extracellular fluids of rat brain tissue especially during freely moving condition.
In order to improve the selectivity of DA sensing, this study of utilizesself-assembly monolayers (SAMs) for providing a simple and convenient method to functionalize the chemical properties of electrode-electrolyte interface. Suchalkanethiol compound constructs the negatively charged monolayers to provide the specific DA detection among competing L-ascorbic acid interferences. Moreover, the large sensing area of three-dimensional gold nanodendrite (Au-DT) is formed by electrodeposition, which possesses good sensitivity and faster electrons for real-time monitoring purposes for microelectrodes. Using amperometric i–t measurement, in vitro tests have shown that the responsive current of the platinum microelectrode coated with Au-DT and 11-mercaptoundecanoic acid (MUA) linearly depends on DA over the range of 0.1–1μM with a sensitivity of 0.377 nA/μM at fast response time of less than 2s.
Furthermore, the dopamine signal can be measured using amperometry technique which has been verified in acute animal model. The developed dopamine system can be transmitted in a wireless model which could be a useful experimental tool for continuous monitoring of dopamine levels for investigating PD treatment in freely moving rat.Our ultimate goal is to apply the wireless miniature DA sensing unit for in-vivo DA recording as a direct evidence and evaluation tool for novel treatment such as transcranial direct current stimulation (tDCS) treatment for PD animal study.
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