| Summary: | 碩士 === 國立成功大學 === 化學系碩博士班 === 101 === The accelerating appearance of drug-resistant bacteria results in a serious threat to human health. As a result, it is urgent for scientist to search for new effective antibiotics.
Currently, some naturally occurring antibacterial agents, such as vancomycin and nisin typed molecules, have been demonstrated not only to show potent antibacterial activity, but also to target peptidoglycan (PG) fragments or peptidoglycan precursors.
In this work, we designed several PG molecules such as the tripeptide and glycopyrophophates to immobilize on a solid-support through a proper linker and conjugation conditions. To approve our concept, these PG-immobilized resins were interacted with natural binders, including vancomycin and crude nisin, to investigate how to enrich or identify PG-binders from crude mixture. Our probes with the assistance of analytical equipment, such as imaging mass spectrometry (IMS) and/or high-performance liquid chromatography (HPLC), become a powerful tool, allowing us not only to explore new PG binders but also to simplify traditional purification procedures.
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