Amperometric sensing of bilirubin via an imprinted polymer modified electrode doped with a room-temperature ionic liquid

• Main Authors: Yan-DiTseng, 曾彥迪
• Other Authors: Mei-Jywan Syu
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/53442131998250107126

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 101 === Bilirubin is the metabolite of human hemoglobin. The concentration of bilirubin in serum is often regarded as an important physiological indicator of liver diseases. The electrochemical sensing of bilirubin was developed the molecularly imprinted polymer film modified electrode doped with room-temperature ionic liquids (RTILs) is investigation of fabricated in this work for the specific binding and electrochemical properties. For the preparation of the imprinted polymer, bilirubin was the template molecule, by using molecular imprinting methacrylic acid (MAA) and ethylene glycol dimethylacrylate (EGDMA) were used as functional monomer and cross-linker, respectively. RTIL, 1-butyl-3-methylimidazolium bis(trifluoro-methylsulfonyl)imide ([BMIM][TF2N]) for the synthesis of bilirubin imprinted polymer film by heated polymerization in the presence of the initiator 2,2-azo-bis-isobutyronitrile (AIBN). The scanning electron microscopic image show that the imprinted polymer film thus synthesized was rather uniform and the average thickness was approximate 1.98 ± 0.03 m。After extraction, a large portion of bilirubin templates was removed. Therefore, the polymer film would be left with cavity specific for the binding of bilirubin. Via which, the electrochemical detection of bilirubin could be possible to carry out. In summary, RTILs may improve the signals of the MIP electrodes. The imprinting factor of 5.52 ± 0.09 and the sensitivity of 0.367 ± 0.019 μA/cm2/mg/dL cound be achieved at the low concentration range (0.2-1.0 mg/dL) from the RTIL-MIP electrode fabricated in this work. The selectivity of bilirubin against biliverdin is 2.13 ± 0.02. For interference test, bilirubin was further detected by the RTIL-MIP electrode in the presence of bovine calf serum (BCS) and the sensitivity was nearly the same as the background to background of water. Thus, it confirms the RTIL-MIP electrode may avoid the interference from serum. The results indicate the feasibility of using the RTIL-MIP electrode for the detection of bilirubin in serum.