Fabrication of NaYF4:Yb, Er/ reduced graphene oxide nanocomposite with NIR upconversion fluorescence imaging and photothermal therapy

• Main Authors: Wen-ChiChang, 張文吉
• Other Authors: Dong-Hwang Chen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/19333196351256241552

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 101 === In this thesis, graphite oxide was synthesized by modified Hummers method and then reduced and surface modified with L-arginine via the microwave method to yield the well-dispersed reduced graphene oxide at first. Secondly, the NaYF4:Yb, Er nanoparticles with near infrared (NIR) upconversion fluorescence imaging property were synthesized, surface coated with silica nanoshells, and then further modified with 3-(triethoxysilyl)propylsuccinic anhydride to generate carboxylic groups on the surface of silica nanoshells. Finally, they were covalently bound on the arginine-modified reduced graphene oxide to form the nanocomposite combining the functions of NIR upconversion fluorescence imaging and photothermal therapy. By transmission electron microscopy (TEM), atomic force microscope (AFM), X-ray diffraction (XRD), Fourier transform infrared spectrometry (FTIR), Raman spectra, electron spectroscopy for chemical analysis (ESCA), UV/VIS/NIR spectrophometer, and fluorescence spectrophotometer, the products’ morphologies, sizes, crystalline structures, and optical properties were characterized. It was found that the formation of reduced graphene oxide, NaYF4:Yb,Er nanoparticles, and their nanocomposite has been achieved successfully. In addition, by using a HeLa cancer cell line, it was demonstrated this nanocomposite indeed possessed both the functions of NIR photothermal therapy and fluorescence imaging.