| Summary: | 碩士 === 國立成功大學 === 化學工程學系碩博士班 === 101 === In recent year, the nanotechnology have been applied in many fields like biomaterial and medical. Especially in magnetic nanoparticles, according to its specific property ‘superparamagnetic’, the magnetic nanoparticles would play an important role as drug carrier to do the in vivo diagnosis. Magnetic nanoparticles would attracted by external magnetic field to the lesions, and it would be more specific if the particle surface binding with active targeting ligand like drug or antibodies.
In this research, the magnetic nanoparticles were synthesized by thermal decomposition to form Fe3O4@OA/OC nanoparticles, then modified by 3-aminopropyltriethoxy silane (APTES) to make the nanoparticles surface covered of amino group to enhance hydrophilicity. Finally we conjugated anti-HbA1c on nanoparticles in order to target HbA1c antigen to monitor diabetes.
Transmission electron microscopy (TEM) images reveals that Fe3O4¬@OA/OC were monodisperse in n-hexane, and its diameter was estimated to be 4.7± 0.7nm, after APTES modified the particles aggregated, the Fe3O4@APTES diameter enlarged to 10.6± 1.3nm. X-ray diffraction (XRD) analysis confirmed that the crystalline plane is the same between Fe3O4@OA/OC ＆ Fe3O4@APTES, thermogravimetric analysis (TGA) observed the weight loss to make sure that APTES were successfully modified on particle surface, Fourier transform infrared spectroscopy (FT-IR) could also approve the fact by the peaks different between Fe3O4@OA/OC and Fe3O4@APTES, finally the magnetization is analyzed by superconducting quantum interference device (SQUID), the saturated magnetization of Fe3O4@OA/OC and Fe3O4@APTES were 39.73 emu/g and 25.23 emu/g respectively.
In biofunctional magnetic nanoparticles section, Fe3O4@APTES conjugated with Anti-HbA1c (Anti-Fe3O4) were identified by FT-IR, there was a special peak shown on the spectrum at 400-500 cm-1 represented antibodies S-S bond. The supernatant which magnetic nanoparticles immune with antibodies was analyzed by ultraviolet-visible (UV/Vis), the results shown that about 4.438 mg Anti-HbA1c conjugated on nanoparticles surface. Finally we did an in vitro test to observe whether the biofunctional particles will traced HbA1c or not, the results of HPLC approved that Anti-Fe3O4 could successfully target to HbA1c, so the biofuctional magnetic nanoparticles could further monitored diabetes patients’ blood HbA1c concentration.
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