Synthesis and characterization of the PEG-based hydrogel in photo-polymerization

• Main Authors: Chih-Chun Chang, 張志鈞
• Other Authors: Shu-Ping Lin
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/pg23yu

碩士 === 國立中興大學 === 生醫工程研究所 === 101 === The tissue engineering and regenerative medicine have been widely studied in recent years because of the speedy development of the medical technology. Cell culture in three-dimensional (3D) environment has been developed in order to mimic the living body environment. 3D matrices have been drawn a lot of attentions because of their customized structures and tailor-made mechanical and chemical properties for cellular proliferation and survival after integrating with the extracellular matrix (ECM) or biomolecules. In this study, 3D polymer scaffold was synthesized by photopolymerization of mixture of 20 % phosphate buffered saline (PBS) and 80% 3D polymer precursor solution with a visible light exposure for 5 min. 3D polymer precursor solution was prepared by mixing poly(ethylene glycol) (PEG) and photosensitive poly(ethylene glycol) diacrylate (PEGDA) with different ratio, including 1:5, 1:3, 1:1 and 3:1. The synthesized PEG-based 3D polymer scaffolds were further characterized by UV/VIS spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), nuclear magnetic resonance spectroscopy (NMR), field emission scanning electron microscopy (SEM), hydrophilic test, mechanical tests, degradable tests, and cell biocompatible tests. The absorbance was proportional to the amount of PEGDA in UV/VIS spectra. All the ratios of PEG-based 3D polymer scaffold exhibited specific functional groups of PEG and PEGDA in ATR-FTIR and NMR spectra. In addition, our characterization of all the ratios of PEG-based 3D polymer showed photopolymerization of PEG and PEGDA due to the absence of C=C bond of PEGDA in ATR-FTIR and NMR spectra. In the ratio of 3:1 of PEG-based 3D polymer scaffold, SEM showed rougher surface with porous structure and the measurement of contact angle displayed smaller angle due to the increase of hydrophilic PEG molecule. In the mechanical tests, the ratio of 1:5 of PEG-based 3D polymer scaffold possessed higher Young’s modulus (6.57±0.14 MPa), break strain (0.64±0.07 MPa) and elongation (24.11±1.37 %). Degradation was quantified by the release of dye of eosin Y and showed relatively higher degradation of 14.33±0.07 % in the ratio of 3:1 after 30 days of incubation. In in-vitro study, porous 3D matrix served as scaffolds for mechanical and structural support to 3T3 fibroblasts and PC12 cells. Cell viability examinations independently show both cell lines healthily thrived in the ratio of 1:5 of PEGDA-peptides supplemented matrices for 5 days. Our in-vitro results suggest PEGDA supplemented with appropriate peptides can introduce biocompatible environments for varied cell growth. These data elucidate that PEG-based 3D polymer scaffold exhibit promisingly mechanical, physical and biological properties for cell growth and are possibly suitable for future large-scale tissue regeneration and tissue replacement.