Correlation Analysis between Plasma Concentration of Indoxyl Sulfate and Clinical Manifestation of Renal Disease in Dogs and Cats

• Main Authors: Ming-Juei Hsieh, 謝明瑞
• Other Authors: Feng-Pang Cheng
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/71957380207979147712

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 101 === Indoxyl sulfate (IS), a protein-bound uremic toxin produced by resident bacteria in the intestine, is accumulated in the serum of human patients suffering from chronic kidney disease (CKD). Free radical induction, nephrotoxicity, cardiovaslular toxicity, and osteoblast cytotoxicity are derived from negative biological effects associated with IS. Although some uremic toxins, especially IS and p-cresyl sulfate, were well studied in human medicine, relevant research is scarce in veterinary field. To investigate whether IS have any association with renal diseases in small animals, a preliminary cross-sectional study on dogs and cats with or without renal disease (RD or non-RD) was performed. RD group was further divided into AKI and CKD groups, by IRIS staging system. Plasma samples obtained from non-RD (64 dogs and 38 cats) and RD (66 dogs and 48 cats) groups, were detected for IS level by using high performance liquid chromatography (HPLC) equipped with fluorescence detection. In addition, plasma samples from two AKI dogs undergoing hemodialysis and from four CKD dogs participating 4-month-long probiotics clinical trial were also collected. Results: the IS and creatinine levels were moderately correlated in RD dogs (ρ=0.60, p<0.05), and only mildly correlated in RD cats (ρ=0.38, p<0.05). IS level was significantly higher in RD and AKI groups as compared to corresponding groups (p<0.05), and was also higher in IRIS stage 4 than lower stages (p<0.05). In two hemodialysis patients, the IS levels reduced largely at the first time (61.6 and 62.4 %), but the efficiency decreased dramatically at the second time of hemodialysis (7.2 and 23.4 %). During probiotics administration period, IS levels decreased in two dogs (2/3). These results may be associated with renal progression, but more cases were needed for deliberate statistical analysis. Conclusively, IS level is significantly associated with the severity of renal disease. IS might be used as an alternative or combined factor to evaluate the progression of renal diseases in companion animal medicine.