The evaluation of different molecular marker systems on genetic diversity analysis of vascular plants.

• Main Authors: Li-Yin Lin, 林俐吟
• Other Authors: 古新梅
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/d43b66

碩士 === 國立中興大學 === 農藝學系所 === 101 === Plant phylogeny could be generated based on morphological marker, isoenzyme, molecular marker and organelle DNAs. Molecular markers are commonly applied in the analysis of genetic diversity among genus or species. Organelle DNAs such as rbcL gene and atpβ gene are better tools for studying analysis of genetic diversity above the order level. It’s necessary to select suitable tool to study the genetic diversity of different population structure. The objective of this study was to evaluate the different molecular marker systems on genetic diversity analysis of vascular plant. Vascular plant in this study was collected from the campus of Chung Hsing University including seed plant and fern. The different molecular marker systems, RAPD, ISSR and SRAP, were selected because of their fast, simple, low cost and without the need of genome information. The result of the dendrograms in this study constructing by molecular markers showed inconsistent to the phylogeny relationship of the seed plant and fern, particularly above the order level. Interestingly, the members of same genus Platycerium spp in the high level were clustered together on the dengrograms of RAPD, ISSR and SRAP analysis. The Matthiola incana belonging to same species were clustered together as well. The genetic diversity of the species of Pinopsida and Pteridophyta from vascular plant were also investing by molecular markers. The result also showed that the species of same families or genus did not cluster together. In conclusion, difference of genome among the high level species and numbers of primer we used were more suitable in studying the genetic diversity below genus level instead of higher levels by molecular marker systems on genetic diversity analysis.