Application of transferable monocot SSR markers for analyzing genetic diversity of Taiwan native Begonia

• Main Authors: Tzu-Chuan Lo, 羅子絹
• Other Authors: Hsin-Mei Ku
• Format: Others
• Language: en_US
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/93599446626197936276

碩士 === 國立中興大學 === 農藝學系所 === 101 === Begonia is the sixth-largest genus of vascular plants in the world. There are fourteen native species and four endemic natural hybrids in Taiwan in which the highest density of natural hybrids and species containing the most complex chromosome numbers in Asia have been reported. Besides, the native Begonia species in Taiwan show great potential as ornamental and medical plants. However, very few molecular tools have been conducted in Begonia species identification and diversity investigation. This study had employed 120 SSR markers designed from monocot genomes to test transferability on Begonia species in Taiwan. An average of 60.6% transferability and multi-unique loci with 100% polymorphism were obtained. In addition, the degree of transferability was influenced by SSR motif types, length and distribution in coding or noncoding regions especially. Simple and imperfect type showed higher transferability. Meanwhile, transferability values of tetra- and dinucleotide repeats were higher than those of tri-nucleotide. Markers derived from coding region revealed higher polymorphism and transferability than those of noncoding region markers. The dendrograms penetrated by data from coding region markers was more similar to total region markers than those of noncoding region markers in diversity analysis. Three groups of dendrograms based on coding, noncoding and total markers were in accordance with previous studies of phylogeny of Begonia species in Taiwan. It demonstrated that SSR markers derived from monocot genomes can be applied in genetic diversity and phylogenetic analysis of distantly related dicotyledons.