Application of monocot SSR and ISSR markers on the genetic diversity analysis of Alpinia species

• Main Authors: Hsiang-Hao Su, 蘇向豪
• Other Authors: Hsin-Mei Ku
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/5ay86z

碩士 === 國立中興大學 === 農藝學系所 === 101 === By 2012, 18 Alpinia species in Taiwan publications have been reported, including A. flabellata、A. formosana、A. galanga、A. intermedia、A. japonica、A. kusshakuensis、A. mesanthera、A. pricei、A. pricei var. sessiliflora、A. shimadae、A. shimadae var. kaeadamii、A. tonrokuensis、A. uraiensis、A. zerumbet、 A. nantoensis、A. koshunesis、A. oui and A. xilanensis. These Alpinia species are classified into four subsections, subsect. Presleia、subsect. Alpinia、subsect. Catimbium and subsect. Cenolophon. However, due to the same chromosome number (2n = 48) and the overlapping of the partial species’ growth regions and flowering periods, these Alpinia have shown the sympatric hybridization and the intermediate hybridization phenomenon. In addition, with the distinct geographic and climatic variations in Taiwan, Alpinia might evolve into different ecology type populations. This causes difficulties in identifying Alpinia species. A. intermedia complex, a name integrated with ‘A. kelungusis、A. suishaensis and A. oblongifolia’ is an example for its distribution throughout wide spread Taiwan and being affected by various environments. In this study, 120 monocot SSR (simple sequence repeat) markers and 17 ISSR (inter simple sequence repeat) markers were performed to investigate the genetic diversity of seventeen Alpinia species and A. intermedia complex collected from18 different geographical regions in Taiwan. In the first part of the tests, 120 monocot SSR markers transferred into 17 Alpinia species, with 64.7% transferability and high distribution ability (PIC = 0.18), and further resulted in classified Alpinia species and the dendrograms, which were consistent to four taxonomically subsections. In the second part, 120 monocot SSR markers transferred into A. intermedia complex showed a higher PIC value (0.14), than that of seventeen ISSR markers analysis (0.26). However, the dendrograms indicated that monocot SSR, ISSR or combined data all grouped A. kelungusis、A. suishaensis and A. intermedia var. intermedia into different clusters. In conclusion, the results suggested that monocot SSR markers transferred system showed a high PIC value and reliable dendrograms on genetic diversity analysis of Alpinia species and could be an efficient tool for genetic diversity studies of species with limited genetic information.