Electrochemical impedance spectroscopy measurement of A-beta(1-42) protein using a nanostructure biochip

• Main Authors: Chun-Che Chiu, 邱俊喆
• Other Authors: 吳嘉哲
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/w32xup

碩士 === 國立中興大學 === 機械工程學系所 === 101 === A-beta (1-42) peptide is one of the possible candidate to predict Alzheimer’s disease. In this study, we demonstrate an EIS based nanostructured biosensor, with uniformly deposited GNPs as the sensing electrode, for effective detection of A-beta (1-42) peptide. An anodic aluminum oxide (AAO) layer with a nanohemisphere array was used as the substrate. A gold thin-film was sputtered onto the AAO substrate to serve as the electrode for GNP deposition and sensing for A-beta (1-42) peptide. A-beta (1-42) antibody was prepared and was verified the specificity with A-beta (1-42) peptide by western blot. We also observed the aggregation of A-beta (1-42) peptide when the concentration is 1 μg/ml. The morphology of A-beta (1-42) was in the form of round aggregations with around 1500-2000 nm diameter. Electrochemical impedance spectroscopy (EIS) measurement for nanostructured biosensors is used to screen the concentration of A-beta (1-42) peptide. The plot for the dependence of EIS concentration measurement resulted in an equation y=29098x+90150 with R2 of 0.9916. The linear detection range existed between 1pg/ml to 10ng/ml of A-beta (1-42).