Ethyl Acetate Extract of Sterculia nobilis Induces Apoptosis of Triple Negative Breast Cancer Cells Through the Overexpression of Apoptosis Inducing Factor
碩士 === 國立中興大學 === 生物化學研究所 === 101 === In previous studies at our laboratory, we found that the Ethyl acetate extract, isolated from shell of pod of Sterculia nobililis (EPSN), can induce the apoptosis of human triple-negative breast cancer cell line BT-20 through the regulation of apoptosis-ind...
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| Format: | Others |
| Language: | zh-TW |
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2013
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| Online Access: | http://ndltd.ncl.edu.tw/handle/79589626957427488929 |
| Summary: | 碩士 === 國立中興大學 === 生物化學研究所 === 101 === In previous studies at our laboratory, we found that the Ethyl acetate extract, isolated from shell of pod of Sterculia nobililis (EPSN), can induce the apoptosis of human triple-negative breast cancer cell line BT-20 through the regulation of apoptosis-inducing factor (AIF) in caspase-independent pathway. In this study, we used EPSN to treat BT-20 cells for cytotoxicity test (48 Hr IC50：40-50 μg/mL) and to investigate how does AIF regulate the mechanism of apoptosis in cancer cells.
The results of Western blotting analysis revealed that, in both BT-20 and MDA-MB-231 cell lines, AIF was started to overexpress in cytosol compartment after 2 hours treatment of EPSN. We further used Real-time PCR to observe the mRNA expression level AIF in BT-20 after the treatment of EPSN. The data showed that there was indeed a signicant increment expressed in mRNA level of AIF after 3 hours treatment of EPSN. These results demonstrated that EPSN can induce apoptosis at the early stage in triple-negative breast cancer cells with the mRNA and protein overexpressions of AIF leading to the cell death through the caspase-independent pathway.
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