| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 101 === Barrett’s esophagus (BE) is precancerous lesion of esophageal adenocarcinoma (EAC). The diagnosis of BE is established by endoscopic suspected esophageal metaplasia (ESEM) and replacement of esophageal squamous epithelium by columnar epithelium with goblet cells (intestinal metaplasia (IM)). However, columnar epithelium from Esophagus or stomach cannot be differentiated by histologic finding. This study was designed to compare the different expression of Transforming growth factor β (TGF-β), a potential biomarker for gastrointestinal carcinogenesis and EAC, in different columnar epithelium. Thirty patients with endoscopic confirmed BE (ESEM) were enrolled. Expression of TGF-β was assessed by reverse transcription-polymerase chain reaction (RT-PCR), western blot and hematoxylin-eosin stain (HE) over biopsy specimens from normal esophagus, BE, columnar epithelium from cardia. Expression of TGF-β was assessed by RT-PCR, western blot over four cell lines (HETA1 (normal esophagus), CP-A (gastric metaplasia (GM)), CP-C (IM) and OE-33 (EAC)). Expression of TGF-β in ESEM with IM and GM were all significantly lower than normal esophagus and stomach. Expression of TGF-β was also lower in cell lines from in EAC and BE with IM and GM than cell lines from normal esophagus. Application of bile acid deoxycholic acid (DCA) resulted to general decrease of TGF-β mRNA in all cell lines. Thus expression of TGF-β may be a potential a biomarker for differentiating columnar epithelium from stomach, normal esophagus to BE.
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