Expression of ATAD3A in ovarian cancer

• Main Authors: Pi-Lien Lu, 呂碧蓮
• Other Authors: Kuan Chih Chow
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/05928004524223770273

碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 101 === ATAD3A (ATPase family, AAA domain-containing 3A) is a kind of mitochondrial transmembrane proteins, attached to the member of the AAA-ATPase family (He et al., 2012). The performance of ATAD3A is closely related to the cell cycle process, while S phase reaches to the maximum amount, the M phase is manifested to the minimum amount. This proves the protein plays the important role in mitochondria. Dihydrodiol dehydrogenase (DDH) belongs to aldehydes and ketones reductase superfamily member (aldo-keto reductase, AKR), the weight of one single monomer molecular is approximately 36 kDa, involved in the metabolism of polycyclic aromatic hydrocarbons detoxification process (Chow et al., 2006), if the DDH is overly expressed in early stage, the tumors and a higher incidence of distant metastasis (Chang et al., 2009), are related to tumor development and prognosis. Therefore, to test the performance of expansion of ovarian cancer with our homemade ATAD3A and DDH monoclonal antibody, the result shows the stained of mitotracker and immunofluorescence, the ATAD3A is existed in mitochondria, and indeed the ovarian cancer cell lines are highly performed, and the percentage of DDH in ovarian cancer cell lines is 33%. In immunohistochemistry(IHC), we found that not all the cancer tumors have a positive coloration, the prediction is that both proteins of ATAD3A and DDH might form a part of the tumor for the performance or non-performance. The Immunohistochemistry can only be qualitative rather than quantitative. In resistance, the result of ovarian follicles can screen the Follicle-stimulating (FSH), luteinizing hormone (LH), progesterone, estradiol and other hormones, whether the relationship between ATAD3A and DDH with chemotherapy drugs (Cisplatin) are associated, worthy of further exploration.