Establishment of multiplication protocols on medicinal plants of Melissa officinalis L. and Ocimum basilicum L.

• Main Authors: Shin-Shien Ho, 何信賢
• Other Authors: Song-Iuan Liaw
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/79535488085227951247

碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 101 === The aims of this study were to establish in vitro multiplication protocol of Melissa officinalis L. and Ocimum basilicum L. The processes include establishing aseptic explants,inducing shoots, rooting and transplanting. In the shoots inducing test, the medium of Melissa officinalis L. were BA 0.1 mg/L＋NAA 0.01 mg/L, BA 0.2 mg/L＋NAA 0.02 mg/L, BA 0.4 mg/L＋NAA 0.04 mg/L,BA 0.6 mg/L＋NAA 0.06 mg/L, BA 0.8 mg/L＋NAA 0.08 mg/L, with BA-NAA ratio 10:1 and the Murashige and Skoog medium (MS) was the control group. In vitro micropropagation of Melissa officinalis L. was established to get maximum shoots multiplication rate by cultivating axillary buds on Murashige and Skoog medium (MS) supplemented with BA 0.1 mg/L+ NAA 0.01 mg/L, but added plant growth regulators suppressing growth of explants.In the rooting test, the medium of Melissa officinalis L. were MS, 1/2 MS, 1/2 MS with IBA 0.2 mg/L, 1/2 MS with IBA 0.5 mg/L, 1/2 MS with NAA 0.2 mg/L and 1/2 MS with NAA 0.5 mg/L. Rooting rate achieved 100.0 % by cultivating on 1/2 MS medium for 1 weeks. In the inducing shoots test, the medium of Ocimum basilicum L. were BA 0.1 mg/L＋NAA 0.01 mg/L, BA 0.2 mg/L＋NAA 0.02 mg/L, BA 0.4 mg/L＋NAA 0.04 mg/L, BA 0.6 mg/L＋NAA 0.06 mg/L, BA 0.8 mg/L＋NAA 0.08 mg/L with BA-NAA ratio 10:1 and the Murashige and Skoog (MS) medium was the control group. In vitro micropropagation of Ocimum basilicum L. was established to get maximum shoots multiplication rate by cultivating axillary buds on Murashige and Skoog (MS) medium supplemented with BA 0.1 mg/L+ NAA 0.01 mg/L. In the rooting test, the medium of Ocimum basilicum L. were MS, 1/2 MS, 1/2 MS with IBA 0.2 mg/L, 1/2 MS with IBA 0.5 mg/L, 1/2 MS with NAA 0.2 mg/L and 1/2 MS with NAA 0.5 mg/L. Rooting rate achieved 100.0 % by cultivating on 1/2 MS for 1 weeks.