Investigation of the role of magnesium ion in the inhibitory effect of magnesium lithospermate B on Na+/K+- ATPase

• Main Authors: Chung-Ju Ho, 何昌儒
• Other Authors: 李豐穎
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/53604670517840463880

碩士 === 國立中興大學 === 化學系所 === 101 === Salvia miltiorrhiza is one of the famous traditional Chinese medicines and is widely used to treat heart disease. It is pointed out in recent literature that magnesium lithospermate B (MLB), the major soluble ingredient of danshen, possess inhibitory activity on Na+/K+-ATPase, and therefore the purification of MLB and its acidic form (lithospermic acid B, LSB)becomes an important issue. However the purification process of MLB reported in literature is time-consuming and expensive. In this study, we developed a simple and rapid method without chromatographic means to obtain LSB in large quantity and high purity through controlling pH value and phase separation. The quantitative analysis of LSB was performed by high-performance liquid chromatograph (HPLC).