| Summary: | 碩士 === 國立中興大學 === 化學工程學系所 === 101 === In recent years, trehalose and gluconic acid are promises to be popular in foodstuff、cosmetology and pharmaceutical industry. It has stability, moisture resistance and low sweetness. With the increase of the trehalose and gluconic acid demand, the need to explore alternative feedstock sources and purification processes that are inexpensive and efficient is becoming more important. Then there have enzyme immobilization method to produces trehalose and gluconic acid from maltose .
This paper reports the purification results of trehalose and gluconic acid by using weak anion exchanger Amberlite IRA-67 and Diaion PA316. Then, explore the anion exchange resin adsorption dynamics and thermodynamics, and then find optimal adsorption conditions. Adsorption isotherm and breakthrough curves for the separation of trehalose and gluconic acid were obtained at pH 2.4 and 7.0, respectively. About the Amberlite IRA67,the isotherm was found to be a Langmuir type at pH 2.4. At pH 2.4, the maximum adsorption capacity of the resin, q and dissociation constant, K were 2.2618(mmole/gwet resin) and 0.0276(mmole/L), respectively. Breakthough time and exhaustion time have been found by breakthrough curve of trehalose and gluconic acid； tB：250min. and tE：475min. And concentration at exhaustion time is 73. 066mM .
Finally ,we found the constant ka (11.23min.-1 )by using equilibrium curve and breakthrough curve,it can be use at big production in the company. When column separation was performed at pH 2.5 by using desorbent (500mM NaCl), the total yield was about 85~95%. Diaion PA316 the isotherm was found not to be Langmuir type at pH 7 .
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