| Summary: | 碩士 === 國立中興大學 === 化學工程學系所 === 101 === In this study, supercritical anti-solvent pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-β-carotenes. Total concentration of 9-cis (134.7) and trans-β-carotene (204.2) increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for cis and 520.8 for trans) of the elution fraction by 82% ethanol. Supercritical anti-solvent (SAS) method produced particulates containing 932.1 mg/g (355.6 for cis and 576.5 for trans) of total β-carotenes with a total recovery of 86.3% (83.8% for cis and 87.8% for trans). Effects of two SAS operational parameters on the precipitated amount, total β-carotene recovery, mean size and morphology of the precipitates were obtained from pre-experiment Subsequently, two-factor response surface method designed SAS precipitation by changing pressure from 90 to 130 bar and time from 10 to 30 min at feed flow rate of 0.6 ml/min, feed concentration of 5 mg/ml, CO2 flow rate of 15 L/min and temperature of 60 °C. It is show that the maximal purity of total-β-carotene obtained from the suitable operation pressure and time in RSM study. And, the mean particle size decreased with the operation pressure, increased with the precipitation time. This work provided an environmental benign process to generate micronized particulates riched with 9-cis and trans-β-carotenes.
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