1. Use feature proteins of Taiwan tea as biomarkers for the certification of origin2. Analysis of hypoglycemic drugs with Fe3O4@graphene oxide nanoclusters

• Main Authors: Han-Ju Chien, 簡涵如
• Other Authors: Chien-Chen Lai
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/22845830515413075318

碩士 === 國立中興大學 === 分子生物學研究所 === 101 === [論文題目一] Oolong tea is one of the major teas in Taiwan, drinking of tea appropriately can prevent cardiovascular disease, obesity and cancer, etc. Recently, the cheap-cost and the poor-quality of the foreign teas have made to imitate Taiwan oolong tea or blend the low-quality foreign teas with Taiwan oolong teas, and sell it at high price; it results in a strong impact on Taiwan oolong teas’ industry. There are origin certification marks to insure the quality of teas grant from intellectual property office in Taiwan, but the judgment is subjective, for instance, the morphology, color, smell, taste, etc. So, it’s need objective scientific basis to judge the teas. So far, several studies used proteomics method to find out the significant expression of proteins, to understand the effects of environments on creatures, the mechanisms of drugs and biomarker discovery, and so on. To assure the integrity of each producing area of oolong tea, 100% original tea can be bought by consumers, and avoid the blending low-quality foreign teas with Taiwan oolong teas, herein, 2-DE and nano-LC-MS/MS was applied to the comparative proteomics analysis of the teas with different production place, to find out the specific proteins as biomarkers for the certification of origins. In this study, we used the spring oolong teas came from 22 different product places to do the proteomics experiments, the protein expression intensity could be measured by software, and used the statistical analysis system to choose the feature protein spots to distinguish the different product places, and then, a database was built by bioinformatics, to establish the platform to   distinguish the oolong teas product places. This platform could be used in forecasting the produce places and gave each tea farmer a product resume; on the other hand, it is safer to consumers, and also decreases the dispute of commerce. [論文題目二] Hypoglycemic drugs can reduce the level of blood sugar; they are used to treat or control the level of blood sugar in diabetes patients, but there is some people uses the drugs to lower the normal blood suger to feel sick to achieve personal purposes. So far, there are many studies using LC-MS/MS to develop rapid methods to analyze Glimepiride, but there has no study showed the analysis of Glimepiride by using MALDI-TOF MS. When analyzing small molecular (m/z < 500) with MALDI-TOF MS, there are many matrix peaks in the area, it will interfere or suppress the signals of small molecules, results in the difficulties of analysis, to overcome the disadvantage, SALDI-TOF MS has been developed, it can resolve the problem. Graphene oxide (GO) is one of the inorganic materials, there was a study reported that they used GO to enrich the long-chain fatty acid, and analyzed with MALDI-TOF MS. Iron oxide nanoparticles (IONP), its superparamagnetism leads no self-aggregate, and it has high surface area to modify with functional groups, and its low rate of precipitation indicates that it’s stable, and there’s no rejection on human body because of its low toxic, good biocompatibility, and it can be apply to biomedicine, the therapy and detection of clinical. There is a study showed that they used the modified IONP to enrich small molecule and analysis with MALDI-TOF MS. Here in, our purpose is that using IO@SLGO, IO@FLGO2-4, IO@FLGO4-8, the different type of nanoclusters, to enrich the hypoglycemic drugs in human urine and analysis it with SALDI-TOF MS, and make comparisons between them.