Determination of tranexamic acid coupled with chemical derivatization by capillary liquid chromatography with ultraviolet detection

• Main Authors: Fang-Yi Liao, 廖芳儀
• Other Authors: Chia-Hsien Feng
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/07645677905464548997

碩士 === 高雄醫學大學 === 香粧品學系碩士班 === 101 === Tranexamic acid is a synthetic derivative of lysine. It is similar to lysine in structure that can use for the inhibition of plasminogen to plasmin, thereby reducing tyrosinase activity and melanin production. In addition to cosmetics uses, tranexamic acid is a medical antifibrinolytic drug used in haemorrhagic conditions and women menstrual blood loses. Because tranexamic acid lacks the chromophore, the derivatization steps are needed for determination of tranexamic acid at low concentration by UV detector. Compared with the previous literature, we chose dansyl chloride as the derivatizing reagent in this study, which had the advantages of the less sample used and stable derivatives. Furthermore we used microwave-assisted reaction to reduce the reaction time which made the operation more quickly. In order to prolong the life time of analytical column, we used dispersive liquid-liquid microextraction (DLLME) to extract excess derivatizing reagent by 4 μL chloroform. Compared with the traditional liquid-liquid extraction, the advantages of DLLME in this study were the less organic solvent used and reduced the waste generated. In this study, we were utilizing capillary liquid chromatography coupling with ultraviolet detector to monitor the dansyl derivative. The dynamic range was from 0.1 to 50 μM with a correlation coefficient 0.999 and the detection limit was 0.03 μM. The precision of intra- and interday were below 0.83 and 3.08%, respectively. Eventually, we were successfully applied this method to determine the content of tranexamic acid in pharmaceuticals, cosmetic products, the stratum corneum extractant and cell samples.