| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所 === 101 === Euphol is a water-insoluble triterpene compound which exhibits the inhibition activity for gastric cancer cell. The purpose of this study was to develop the optimal liposome formulation as a carrier to overcome the poor solubility as well as irritation, and improve the efficiency of euphol. The liposomal dosage form after formulation designing was screened by particle size, polydispersity index, zeta potential, encapsulation efficiency, the image of appearance using TEM and stability. Besides, the bioactivity of formulations was evaluated using MKN45 and CS12 cell viability. At the same time, we also discussed and found the pathway of euphol. The results were shown that the positive-charge liposome formulations containing Epikuron-200/cholesterol/stearylamine = 8/1/0.1 as lipid phase, 1% Tween 80 as water phase had clear appearance and good physicochemical stability. The particle size was about 75~85 nm and the encapsulation rate was almost 100%. Compare to the same concentration solution type and non-charge liposome formulations of euphol, it had the best inhibition activity to gastric cancer cell line. In addition, according to the extraction of gastric cancer cell membrane and affinity detection by the Biacore 3000 interaction analysis system, we inferred that euphol was a drug combining cell membrane easily.
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