| Summary: | 碩士 === 高雄醫學大學 === 醫藥暨應用化學研究所 === 101 === This study intends to develop versatile of the tumorous targeting nanocarrier that
also has to carry cancer drugs, tumorous targeting and nanocarrier image and so on.
This tumorous targeting nanocarrier composed of a near-infrared fluorescence (nearIR) and computed tomography (CT) imaging system for tracking nanogold clusters (AuNCs) grafted on poly(lactic-co-glycolic acid) (PLGA) into the end of the copolymer PLGA PLGA-AuNC. PLGA was grafted with PEG (polyethylene glycol)to PLGA-mPEG copolymer, and by mixed in different proportions of both precipitation to complex nanoparticles. Therefore, there are objectives of the
experiment. Synthesis and analysis near-infrared of AuNCs. (2) Synthesis and Characterization of PLGA-AuNCs. (3) Synthesis and Characterization ofPLGAPEG. (4) By PLGA-AuNC with PLGA-mPEG mixed in differentproportions of both precipitation to complex nanoparticles and evaluated its characteristics and nanoparticle coated with the ability to carry drugs. (5) To evalute the versatile of this nanocarrier for tracking capatibility of tumorous targeting image, and combined with anti-PEG antibodies identificated with target capacity of PEG. The near-IR AuNCs is synthesized by ((±)-α-Lipoamide) as template, and used both amine group end of (±)-α-Lipoamide and carboxyl group end of the PLGA to confenstionreaction to amide bond. The PLGA-AuNC and PLGA-mPEG chemical structure evaluted by the nuclear magnetic resonance (NMR) and infrared spectroscopy (FTIR) , while the nanoparticle size distribution measurement were used theZetaSize analyzed complex nanoparticles, and morphalogy observed bytransmission electron microscopy (TEM). In vitro test, cell viability evaluated by MTT assaywith HeLa cell line and 3T3 cell line. The tumor cell phagocytosis test was used forHeLa3 cell lines, phagocytosis test designed that combined with fluorescent agent (FITC) as the mimic drugs, via laser confocal microscopy and phagocytic propertiesassay that confirmed complex nanoparticles carry with lipophilic drugs capasity and near-IR fluorescence tracking assessments. In vivo test that uses of molecular imaging system (Caliper IVIS system), this complex nanoparticles injected into mice(how old) for evaluted capatibility of fluorescence among AuNCs and CT. Primary experiment results that indicated by NMR and FTIR, the chemical structure of PLGA-AuNC and PLGA-mPEG were synthesized. Particle size analysis of this complex nanoparticle was about the range of 100 ~ 120 nm. TEM observation indicated AuNCs distributed amongcomplex nanoparticles. Cell viability resultthat the nanoparticles were notn-cytotoxic for Hela and 3T3 cell line, and in Confocalobservation, confirmed this nanoparticles can effectively carried withlipophilic fluorescent agent into HeLa cell line with AuNC in near-IR tracking capability. In this result comfirmed that the tumor cells therapeutic potential in the future. In IVIS fluorescence imaging, it is also confirmed that this compound in vivo fluorescent nanoparticles with PEG antibody tumor target organism capibility and near-IR tracking imaging ability. In CT images that has been indicated in vitro with high concentrations of contrast, but the AuNCs preliminary experimental organism because the concentration of AuNCs was too low, yet there is a significant differeance capabilities. In this study that has been successfully synthesized the highbiocompatibility and versatility of the tumorous targeting nanocarrier, that is also carry lipophilic oil-soluble drugs, PEG antibody trageting and nanocarrier tumor near-infrared light, CT images tracking and other functions.
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