| Summary: | 碩士 === 高雄醫學大學 === 天然藥物研究所 === 101 === The antioxidant activity of seaweed extract against free radicals by arecoline was validated in an Adenosine Triphosphate assay (ATP assay), ATP assay. However, the protective mechanism for extracts remains unclear. In this study, we investigated on the protective effects of seaweed extract on in vitro for a Ca9-22 cell line (oral squamous cell cancer cell line, OSCC) in vitro. The DNA damage agent arecoline was used and tested by an ATP assay. ATP content of the arecoline (0.1、0.25、0.35、0.5 mM) treatment was reduced in a dose-dependent manner, but increased by which pre-treated with seaweed extract (0.1、0.25、0.35、0.5 mg/ml). Added arecoline (0.1、0.25、0.35、0.5 mM) to the Ca9-22 cell culture showed that the doses-dependent manner in reactive oxygen species (ROS) and reactive nitrogen species (RNS) were reduced by which pre-treated with seaweed extract (0.1、0.25、0.35、0.5 mg/ml). In treatment with arecoline (0.1、0.25、0.35、0.5 mM), the results of PI with annexin V double stain showed that cell apoptosis and necrosis increased remarkably. The extract (0.1、0.25、0.35、0.5 mg/ml) was effective protected arecoline (0.1、0.25、0.35、0.5 mM) induced annexin V positive that indicated the cell death rate. We expected that arecoline (0.1、0.25、0.35、0.5 mM) induced cell damage prevented by seaweed extract (0.1、0.25、0.35、0.5 mg/ml) was available. Thus, the crude extract of seaweed extract (0.1、0.25、0.35、0.5 mg/ml) has the potential of cell protective abilities against the arecoline (0.1、0.25、0.35、0.5 mM) induced cell damage and become a potential natural product for human health.
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