| Summary: | 碩士 === 高雄醫學大學 === 天然藥物研究所 === 101 === Acmella oleracea (AO) is an annual herb native to the tropical parts of Africa and South America with a yellow flower head, which is commonly used in traditional folk medicine to cure toothaches, throat complaints, and stomatitis. Alkylamides, one category of AO major active components, are regarded for their diuretic, antibacterial, and anti-inflammatory activities. Besides the medicinal use, alkylamides are well-known for their anti-wrinkle activity and used in different skin care products. Despite the extensive use in cosmetic industry, the production and marketing of AO and its alkylamides face a critical threat due to the lack of a reliable quality assessment method to evaluate concentration of alkylamides.
In this investigation, we report a convenient method for concentrating the alkylamide-rich fraction (ARF), from the ethanolic extract of AO flowers (EEF-AO). Using bio-activity guided isolation technique, five alkylamides, including spilanthol (3), (2E,6Z,8E)-N-(2-methylbutyl)-2,6,8-decatrienamide (4), (2E,5Z)-N-isobutylundeca-2,5-diene-8,10-diynamide (5), (2E)-N-isobutyl-2-undecene-8,10-diynamide (6), (2E)-N-(2-methylbutyl)-2-undecene-8,10-diynamide (7), and a mixture of steroids 1-2, compound 5 is new. To detect the concentration of these markers in different plant parts, a rapid HPLC-PDA method for simultaneous detection of alkylamides from AO and A. ciliata (AC) was established. Two major alkylamides 3 - 4 were separated as the chemical markers of ARF. Using the developed HPLC-PDA protocol, the contents differences of major alkylamides in different part of AO and AC were achieved.
Utilizing the efficient extraction, concentration and rapid analysis method not only provide necessary information about AO alkylamides concents in different plants parts but also provide a quality control assessment of AO alkylamides during different harvesting times.
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