| Summary: | 碩士 === 義守大學 === 機械與自動化工程學系 === 101 === This work successfully developed a three-dimensional electrodeless dielectrophoresis microfluidic chip and to be demonstrated the feasibility of the submicro particle trapping and separation approach utilizing an inverse pyramid micro-channel. In this study, experimental observations were facilitated to investigate the phenomena of electrokinetics, i.e., electroosmosis (EO) or dielectrophoresis (DEP) by tuning of the driving frequencies and the applied electric field. These results of EO flow or DEP effect were depended on the interaction of the electroosmosis and dielectrophoresis by tuning of the driving frequency and the applied voltage. That not only revealed the fluid could be generated an electroosmotic flow, but also categorized the optimum trapping/separating parameters for the different particle sizes. Besides, the proposed operating mode can be used to trap/separate the 0.2- and 0.5-μm particles and simultaneously convey them through the microfluidic device, eliminating the need for an additional micropump. Certainly, the device can be envisioned as a single automated device that supports the multi-functions such as transportation, separation, and detection, facilitating the realization of a Lab-on-a-Chip.
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