The Preparation of Rhodobacter Sphaeroides-imprinted Polymeric Thin Film Coated Electrodes for Biofuel Cells

• Main Authors: LI, Minghuan, 李明桓
• Other Authors: Lee, Meihwa
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/82775692322406236662

碩士 === 義守大學 === 材料科學與工程學系 === 101 === In this work, Rhodobacter sphaeroides are imprinted onto Poly(ethylene-co-vinyl alcohol)s, EVALs, containing ethylene from 27 to 44 mole%. At low imprinting cellular concentration, the recognition abilities of imprinted polymers are decreased dramatically with increasing ethylene concentration. In contrast, the adsorption of Rhodobacter sphaeroide achieves as high as 2x107 cells/cm2 when the imprinting and adsorption concentrations are 1x108 and 1x109 cells/mL, respectively. Interestingly, the larger imprinted cavities are found both at lower polymeric solution (5 wt%) or at high ethylene containing EVALs (44 mol%). The reusability for the Rhodobacter sphaeroide-imprinted EVAL thin films is more than 30 times. Finally, the Rhodobacter sphaeroide-imprinted polymeric thin films are used to assemble biofuel cells. The open-circuit voltage (OCV) of this fuel cell is measured as 0.5 V and maintained at least 72 h. Moreover, the maximum power intensity is found at 1.85 mW/m2. The hydrogenase expression of the Rhodobacter sphaeroide is about 2.3-fold under red light higher than that under white light irradiation. The Raman microscopy images also show that the cytochrome b6f complex amount is about 1.8-fold higher when incubated on MIPs than on the NIPs after 72hr. The Rhodobacter sphaeroide-imprinted polymeric thin film coated electrodes may potentially use for the immobilization of bacteria and energy conversion.