Study on Growth Inhibition and Induction of Apoptosis by Methanol Extract of Antrodia Camphorata in MCF-7 Breast Cancer Cells

• Main Authors: Hsin-Che Wu, 吳欣哲
• Other Authors: Tai-An Chiang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/94603039113059825392

碩士 === 中華醫事科技大學 === 醫學檢驗生物技術系碩士班 === 101 === In Taiwan, Antrodia camphorata is a very famous Chinese traditional medicine, in recent years, studies have pointed out that it has antioxidant and anti-cancer function. Taiwan Antrodia camphorata, however, recently has been applied in traditional Chinese medicine and used to treat food poisoning, diarrhea, high blood pressure and liver cancer, but very few biological activity test report. In recent years, the relationship between apoptosis and cancer are quite a lot of attention, more and more studies have shown that tumor transformation, progression and metastasis process involves changing the normal apoptotic pathway. Breast cancer is the most common malignancy in Taiwan women. Approximately one-third of all women with breast cancer develops metastases and ultimately expires due to the effects of the disease. Despite the fact that many tumors initially respond to chemotherapy, breast cancer cells can subsequently survive and gain resistance to the treatment. Breast cancer, like other cancers, occurs because of an interaction between the environment and a defective gene. Normal cells divide as many times as needed and stop. They attach to other cells and stay in place in tissues. Cells become cancerous when mutations destroy their ability to stop dividing, to attach to other cells and to stay where they belong. In the United States, 10-20% of breast and ovarian cancer patients, one or two relatives with these diseases, these cancers familial tendency, known as hereditary breast cancer - ovarian cancer syndrome. Antrodia camphorata (5-20 μg / ml) the cultured MCF-7 breast cancer cells with different concentrations of the MCF-7 cells undergo apoptosis, chromatin condensation, and even DNA sequence fragment accumulation of sub G1 stage.Further, in the process of apoptosis in MCF-7 breast cancer cells, will be accompanied by cytochrome c activation Caspase 3 of release, and PARP degradation.Studies have shown that Antrodia camphorata through induction of apoptosis, inhibit the growth of MCF-7 breast cancer cells, it may be the high value products with anti-cancer drugs.